Antigen

Antigen Class

TFs and others

Antigen

GLI2

Cell type

Cell type Class

Bone

Cell type

Chondrosarcoma

MeSH Description

A slowly growing malignant neoplasm derived from cartilage cells, occurring most frequently in pelvic bones or near the ends of long bones, in middle-aged and old people. Most chondrosarcomas arise de novo, but some may develop in a preexisting benign cartilaginous lesion or in patients with ENCHONDROMATOSIS. (Stedman, 25th ed)

Sample

source_name

chondrosarcoma

tissue

chondrosarcoma

culture

primary cells

treatment

Shh ligand

duration

48 hours

chip antibody

anti-Gli2 (R&D catalog number AF3526)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Samples were subjected to chromatin immunoprecipitation and prepared for sequencing using the Active Motif ChIP-IT kit, according to the manufacturer's instructions. Libraries were prepared according to the Illumina protocol at The Centre for Applied Genomics at The Hospital for Sick Children.

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

hg38

Number of total reads

33734449

Reads aligned (%)

82.2

Duplicates removed (%)

94.5

Number of peaks

269 (qval < 1E-05)

hg19

Number of total reads

33734449

Reads aligned (%)

81.3

Duplicates removed (%)

96.0

Number of peaks

259 (qval < 1E-05)